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e coli genotypes dh5 α  (New England Biolabs)


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    New England Biolabs e coli genotypes dh5 α
    Representation of immunoblot analysis of the cell lysate of <t>E.</t> <t>coli</t> expressing the SCC and GAC pRha and carrying an empty control plasmid (−ve). A) Blot was incubated with PlyCB-Alexa Fluor ® 647 (PlyCB WT AF647 ). B) Probing the same samples with the GAC antibodies confirms the presence of GAC in the bands. Molecular mass markers are given in kDa.
    E Coli Genotypes Dh5 α, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli genotypes dh5 α/product/New England Biolabs
    Average 96 stars, based on 1545 article reviews
    e coli genotypes dh5 α - by Bioz Stars, 2026-02
    96/100 stars

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    1) Product Images from "Molecular basis for recognition of the Group A Carbohydrate backbone by the PlyC streptococcal bacteriophage endolysin"

    Article Title: Molecular basis for recognition of the Group A Carbohydrate backbone by the PlyC streptococcal bacteriophage endolysin

    Journal: bioRxiv

    doi: 10.1101/2021.03.19.436156

    Representation of immunoblot analysis of the cell lysate of E. coli expressing the SCC and GAC pRha and carrying an empty control plasmid (−ve). A) Blot was incubated with PlyCB-Alexa Fluor ® 647 (PlyCB WT AF647 ). B) Probing the same samples with the GAC antibodies confirms the presence of GAC in the bands. Molecular mass markers are given in kDa.
    Figure Legend Snippet: Representation of immunoblot analysis of the cell lysate of E. coli expressing the SCC and GAC pRha and carrying an empty control plasmid (−ve). A) Blot was incubated with PlyCB-Alexa Fluor ® 647 (PlyCB WT AF647 ). B) Probing the same samples with the GAC antibodies confirms the presence of GAC in the bands. Molecular mass markers are given in kDa.

    Techniques Used: Western Blot, Expressing, Plasmid Preparation, Incubation

    PlyCB binding to E. coli cells were investigated by flow cytometry after labelling with PlyCB WT AF647 and PlyCB R66E AF647 mutant proteins. Blue: −ve control cells without pRha. Red: pRha producing E. coli cells. Representative histograms are shown. A) Left panel: unstained cells. Right panel: The anti-GAC antibodies (GAC-FITC) were used as a positive control to label the E. coli cells producing pRha. The antibodies do not bind to the E. coli cells carrying an empty plasmid (−ve).B) Left panel: PlyCB WT AF647 binds to the E. coli cells producing pRha, but not to the E. coli cells carrying an empty plasmid (−ve). Right panel: PlyCB R66E AF647 does not binds to the E. coli cells producing pRha.
    Figure Legend Snippet: PlyCB binding to E. coli cells were investigated by flow cytometry after labelling with PlyCB WT AF647 and PlyCB R66E AF647 mutant proteins. Blue: −ve control cells without pRha. Red: pRha producing E. coli cells. Representative histograms are shown. A) Left panel: unstained cells. Right panel: The anti-GAC antibodies (GAC-FITC) were used as a positive control to label the E. coli cells producing pRha. The antibodies do not bind to the E. coli cells carrying an empty plasmid (−ve).B) Left panel: PlyCB WT AF647 binds to the E. coli cells producing pRha, but not to the E. coli cells carrying an empty plasmid (−ve). Right panel: PlyCB R66E AF647 does not binds to the E. coli cells producing pRha.

    Techniques Used: Binding Assay, Flow Cytometry, Mutagenesis, Positive Control, Plasmid Preparation



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    e coli genotypes dh5 α  (New England Biolabs)
    96
    New England Biolabs e coli genotypes dh5 α
    Representation of immunoblot analysis of the cell lysate of <t>E.</t> <t>coli</t> expressing the SCC and GAC pRha and carrying an empty control plasmid (−ve). A) Blot was incubated with PlyCB-Alexa Fluor ® 647 (PlyCB WT AF647 ). B) Probing the same samples with the GAC antibodies confirms the presence of GAC in the bands. Molecular mass markers are given in kDa.
    E Coli Genotypes Dh5 α, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli genotypes dh5 α/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    e coli genotypes dh5 α - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier

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    Representation of immunoblot analysis of the cell lysate of E. coli expressing the SCC and GAC pRha and carrying an empty control plasmid (−ve). A) Blot was incubated with PlyCB-Alexa Fluor ® 647 (PlyCB WT AF647 ). B) Probing the same samples with the GAC antibodies confirms the presence of GAC in the bands. Molecular mass markers are given in kDa.

    Journal: bioRxiv

    Article Title: Molecular basis for recognition of the Group A Carbohydrate backbone by the PlyC streptococcal bacteriophage endolysin

    doi: 10.1101/2021.03.19.436156

    Figure Lengend Snippet: Representation of immunoblot analysis of the cell lysate of E. coli expressing the SCC and GAC pRha and carrying an empty control plasmid (−ve). A) Blot was incubated with PlyCB-Alexa Fluor ® 647 (PlyCB WT AF647 ). B) Probing the same samples with the GAC antibodies confirms the presence of GAC in the bands. Molecular mass markers are given in kDa.

    Article Snippet: E. coli genotypes DH5 α (NEB, cat. No. C2988J), DH10 α (Thermo Fisher, cat No. 18297010), BL21 (NEB, cat No. C2530H) and Origami 2 (Millipore Sigma, cat. No. 71346) were used for routine plasmid propagation or protein expression and grown in Lysogeny Broth (LB) medium supplemented with either 50 µg/ml kanamycin, 100 µg/ml ampicillin or 35 µg/ml chloramphenicol as needed.

    Techniques: Western Blot, Expressing, Plasmid Preparation, Incubation

    PlyCB binding to E. coli cells were investigated by flow cytometry after labelling with PlyCB WT AF647 and PlyCB R66E AF647 mutant proteins. Blue: −ve control cells without pRha. Red: pRha producing E. coli cells. Representative histograms are shown. A) Left panel: unstained cells. Right panel: The anti-GAC antibodies (GAC-FITC) were used as a positive control to label the E. coli cells producing pRha. The antibodies do not bind to the E. coli cells carrying an empty plasmid (−ve).B) Left panel: PlyCB WT AF647 binds to the E. coli cells producing pRha, but not to the E. coli cells carrying an empty plasmid (−ve). Right panel: PlyCB R66E AF647 does not binds to the E. coli cells producing pRha.

    Journal: bioRxiv

    Article Title: Molecular basis for recognition of the Group A Carbohydrate backbone by the PlyC streptococcal bacteriophage endolysin

    doi: 10.1101/2021.03.19.436156

    Figure Lengend Snippet: PlyCB binding to E. coli cells were investigated by flow cytometry after labelling with PlyCB WT AF647 and PlyCB R66E AF647 mutant proteins. Blue: −ve control cells without pRha. Red: pRha producing E. coli cells. Representative histograms are shown. A) Left panel: unstained cells. Right panel: The anti-GAC antibodies (GAC-FITC) were used as a positive control to label the E. coli cells producing pRha. The antibodies do not bind to the E. coli cells carrying an empty plasmid (−ve).B) Left panel: PlyCB WT AF647 binds to the E. coli cells producing pRha, but not to the E. coli cells carrying an empty plasmid (−ve). Right panel: PlyCB R66E AF647 does not binds to the E. coli cells producing pRha.

    Article Snippet: E. coli genotypes DH5 α (NEB, cat. No. C2988J), DH10 α (Thermo Fisher, cat No. 18297010), BL21 (NEB, cat No. C2530H) and Origami 2 (Millipore Sigma, cat. No. 71346) were used for routine plasmid propagation or protein expression and grown in Lysogeny Broth (LB) medium supplemented with either 50 µg/ml kanamycin, 100 µg/ml ampicillin or 35 µg/ml chloramphenicol as needed.

    Techniques: Binding Assay, Flow Cytometry, Mutagenesis, Positive Control, Plasmid Preparation